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Plasmic region; (ii) pCMV3gL-1 (9), which encodes the entire UL1(gL) open reading frame; and (iii) pX343, which confers resistance to hygromycin B (1). Transfected cells were grown in DMEM in the presence of hygromycin B, the surviving cells were expanded, and clones were obtained from single cells. The supernatant from each clone was screened by Western blot analysis using R83 (anti-gH) and UL1-2

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