Ynthesized with random hexamers after rRNA depletion. Size-selected cDNA fragments (250?00 bp range) were sequenced on an Illumina HiSeq 2000 from both ends. Overall, two sequencing runs were performed yielding 10?0 million 100 bp read pairs per sample. Quality of the reads was analyzed with FastQC v. 0.10.1 (http://www.bioinformatics.babraham.ac.uk/projects/ fastqc) and necessary filtering steps
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