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Supernatant from a 10-ml THB overnight culture. The plasminspecific chromogenic substrate was added, and the mixture was incubated at 37 . After 3 h, the absorbance at 405 nm was measured. Instead of the supernatant, 70 l of medium or 30 l of pSK (10 U/ l) served as negative and positive controls, respectively. Electrophoresis and Western blot analysis. Sodium dodecyl sulfatepolyacrylamide gel ele

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