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The basic Elisa principle and procedure is not much different from other immunoassay technologies. Sandwich Elisa test is used to detecting specific antigen in the unknown sample.

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme.

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