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Ntrifugation at 30,000 g for 5 h through a 10 -30 -60 sucrose-PBS step gradient. The virus band located at the 30 -60 sucrose interface was collected, titered, and stored at 80 . Soluble HSV glycoproteins and infected cell extracts used. Soluble gD1(306t) was produced in baculovirus-infected Sf9 cells and was purified as previously described (52). Cytoplasmic extracts of HSV-1(NS) (16)- or HSV-

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Icle or scab to a maximum score of 5. Swelling and lesions in locations separate from the inoculation site were considered to be secondary or zosteriform disease. Scoring of these lesions was the same as for the inoculation site except that a daily maximal score of 10 was used.stimulate production of neutralizing antibody and affords good protection to mice challenged with HSV-1 in a zosteriform

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Nm (expressed as millioptical density units [mOD]). (A) Blocking of virus entry with purified gCt (OE), gDt (s), or gHt-gL (F); (B) blocking of virus entry with gDt alone (s), gHt-gL (F), or a mixture of 40 nM gD (concentration which gave 50 inhibition of entry) with various concentrations of gHt-gL (!).FIG. 5. Immunoblot (Western blot) analysis of serum samples from rabbits immunized with gHt-g